National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Množení česneku kuchyňského - jarního a ozimého nepaličáku - v podmínkách in vitro
Kozák, Václav
In this Bachelor's thesis we studied influencing of bulbs multiplications growing in vitro by adding grow-regulators PP 333, CEPA, ABA to the medium or increase of sucrose concentration in the medium. The culture from segments of inner clove part was cultivation on primary MS medium and 0.5mg/l GA3 was added and 2iP was replaced by the similar concentration of BAP for the cultures from apical meristems. The experiment was made with 2 unregistered garlic genotype with working names Emilia and 'Japo'. We also observed CO2 and ethylene production by the growing plants and influencing bulbs multiplications by these gases. Explantats made from segments of inner clove part after ribavirin application were tested if they contains OYDV virus and DNA analysis was made in order that we could verify genetic stability of in vitro growth plants. In this work were found statistically significant differences in the production of onions in the individual genotypes of garlic on tested media.

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